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mitotracker deep red  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mitotracker deep red
    Mitotracker Deep Red, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ensa/ENSA+Antibody/pm41764738-152-15-18
    Average 94 stars, based on 18 article reviews
    mitotracker deep red - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: Characterization of Mast2 Kinase Defines Structural Features, Regulation, and Substrates.
    Article Snippet: 26 27 METHODS 28 29 Materials 30 γ-[P32]-ATP was purchased from PerkinElmer Life Sciences.

    Western Blot:

    Article Title: Discovery and Characterization of a Novel MASTL Inhibitor MKI-2 Targeting MASTL-PP2A in Breast Cancer Cells and Oocytes
    Article Snippet: .. Western blotting was performed as described [ , ] using the following antibodies: rabbit polyclonal antibodies against MASTL (Abgent, San Diego, CA, USA); phospho-ENSA (Ser67)/ARPP19 (Ser62), ENSA, cleaved PARP (Asp214), AKT, phospho-Chk2 (Thr68) (Cell Signaling Technology, Danvers, MA, USA); rabbit monoclonal antibody against phospho-c-Myc (Ser 62) (Abcam, Cambridge, UK); mouse monoclonal antibody against caspase-2 (Cell Signaling Technology, Danvers, MA, USA), and c-Myc (Santa Cruz Biotechnology, Inc., Dallas, TX, USA); and a mouse polyclonal antibody against β-actin (Santa Cruz Biotechnology, Inc.). .. The PP2A activity assay was performed as previously described [ ], and the PP2A phosphatase assay according to the manufacturer’s protocol (RediPlate 96 EnzChek serine/threonine phosphatase assay kit; Invitrogen).

    Article Title: Discovery and Characterization of a Novel MASTL Inhibitor MKI-2 Targeting MASTL-PP2A in Breast Cancer Cells and Oocytes.
    Article Snippet: .. Western blotting was performed as described [30,32] using the following antibodies: rabbit polyclonal antibodies against MASTL (Abgent, San Diego, CA, USA); phosphoENSA (Ser67)/ARPP19 (Ser62), ENSA, cleaved PARP (Asp214), AKT, phospho-Chk2 (Thr68) (Cell Signaling Technology, Danvers, MA, USA); rabbit monoclonal antibody against phospho-c-Myc (Ser 62) (Abcam, Cambridge, UK); mouse monoclonal antibody against caspase-2 (Cell Signaling Technology, Danvers, MA, USA), and c-Myc (Santa Cruz Biotechnology, Inc., Dallas, TX, USA); and a mouse polyclonal antibody against β-actin (Santa Cruz Biotechnology, Inc.). .. The PP2A activity assay was performed as previously described [30], and the PP2A phosphatase assay according to the manufacturer’s protocol (RediPlate 96 EnzChek serine/threonine phosphatase assay kit; Invitrogen).

    SDS Page:

    Article Title: Pharmaceutical composition for preventing or treating cancer, containing N-1H-benzimidazol-2-yl-3-(1H-pyrrole-1-yl)benzamide as active ingredient
    Article Snippet: .. 20 μg of protein was separated by SDS-PAGE electrophoresis and transferred to a PVDF membrane, and the following specific antibodies were used: rabbit polyclonal antibody MASTL (Abgent); phospho-ENSA (Ser67)/ARPP19 (Ser62) (#5240), ENSA (#11915S), cleaved PARP (Asp214) (#9541), AKT (#9272), phospho-AKT (ser473) (#9271), phospho-GSK-3α/β (Ser21/9) (#9331), phospho-p70S6K (The 288) (#9205) and phospho-Chk2 (Thr68) (#2661; Cell Signaling Technology, MA); p70S6K (sc-230; Santa Cruz Biotechnology, CA, USA), phospho-H3 (S10) (06-570, Merck, NJ); mouse monoclonal antibody caspase-2 (#2224S). .. In addition, mouse polyclonal antibody β-actin (C4; Santa Cruz Biotechnology, CA, USA) was used as a loading control, it was washed with 1×PBS/0.1% Tween 20, and the binding protein was detected using HRP-binding secondary antibody and improved chemiluminescence detection system.

    Electrophoresis:

    Article Title: Pharmaceutical composition for preventing or treating cancer, containing N-1H-benzimidazol-2-yl-3-(1H-pyrrole-1-yl)benzamide as active ingredient
    Article Snippet: .. 20 μg of protein was separated by SDS-PAGE electrophoresis and transferred to a PVDF membrane, and the following specific antibodies were used: rabbit polyclonal antibody MASTL (Abgent); phospho-ENSA (Ser67)/ARPP19 (Ser62) (#5240), ENSA (#11915S), cleaved PARP (Asp214) (#9541), AKT (#9272), phospho-AKT (ser473) (#9271), phospho-GSK-3α/β (Ser21/9) (#9331), phospho-p70S6K (The 288) (#9205) and phospho-Chk2 (Thr68) (#2661; Cell Signaling Technology, MA); p70S6K (sc-230; Santa Cruz Biotechnology, CA, USA), phospho-H3 (S10) (06-570, Merck, NJ); mouse monoclonal antibody caspase-2 (#2224S). .. In addition, mouse polyclonal antibody β-actin (C4; Santa Cruz Biotechnology, CA, USA) was used as a loading control, it was washed with 1×PBS/0.1% Tween 20, and the binding protein was detected using HRP-binding secondary antibody and improved chemiluminescence detection system.

    Membrane:

    Article Title: Pharmaceutical composition for preventing or treating cancer, containing N-1H-benzimidazol-2-yl-3-(1H-pyrrole-1-yl)benzamide as active ingredient
    Article Snippet: .. 20 μg of protein was separated by SDS-PAGE electrophoresis and transferred to a PVDF membrane, and the following specific antibodies were used: rabbit polyclonal antibody MASTL (Abgent); phospho-ENSA (Ser67)/ARPP19 (Ser62) (#5240), ENSA (#11915S), cleaved PARP (Asp214) (#9541), AKT (#9272), phospho-AKT (ser473) (#9271), phospho-GSK-3α/β (Ser21/9) (#9331), phospho-p70S6K (The 288) (#9205) and phospho-Chk2 (Thr68) (#2661; Cell Signaling Technology, MA); p70S6K (sc-230; Santa Cruz Biotechnology, CA, USA), phospho-H3 (S10) (06-570, Merck, NJ); mouse monoclonal antibody caspase-2 (#2224S). .. In addition, mouse polyclonal antibody β-actin (C4; Santa Cruz Biotechnology, CA, USA) was used as a loading control, it was washed with 1×PBS/0.1% Tween 20, and the binding protein was detected using HRP-binding secondary antibody and improved chemiluminescence detection system.



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    MASTL regulates mitotic catastrophe via the PP2A‐B55α pathway. (A) ENSA and phospho‐ENSA <t>(Ser67)</t> expression after MASTL modulation. (B) PP2A‐B55α protein levels following MASTL perturbation. (C) PP2A phosphatase activity in MASTL‐modified cells. (D) Validation of PP2A‐B55α knockdown efficiency by WB. (E–G) Rescue of MASTL depletion phenotypes by PP2A‐B55α co‐knockdown: (E) Cell viability (CCK‐8), (F) Mitotic catastrophe (α‐tubulin/DAPI), and (G) Paclitaxel response.
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    Image Search Results


    MASTL regulates mitotic catastrophe via the PP2A‐B55α pathway. (A) ENSA and phospho‐ENSA (Ser67) expression after MASTL modulation. (B) PP2A‐B55α protein levels following MASTL perturbation. (C) PP2A phosphatase activity in MASTL‐modified cells. (D) Validation of PP2A‐B55α knockdown efficiency by WB. (E–G) Rescue of MASTL depletion phenotypes by PP2A‐B55α co‐knockdown: (E) Cell viability (CCK‐8), (F) Mitotic catastrophe (α‐tubulin/DAPI), and (G) Paclitaxel response.

    Journal: Cancer Science

    Article Title: MASTL Promotes Hepatocellular Carcinoma Progression and Paclitaxel Resistance Through Mitotic Catastrophe

    doi: 10.1111/cas.70287

    Figure Lengend Snippet: MASTL regulates mitotic catastrophe via the PP2A‐B55α pathway. (A) ENSA and phospho‐ENSA (Ser67) expression after MASTL modulation. (B) PP2A‐B55α protein levels following MASTL perturbation. (C) PP2A phosphatase activity in MASTL‐modified cells. (D) Validation of PP2A‐B55α knockdown efficiency by WB. (E–G) Rescue of MASTL depletion phenotypes by PP2A‐B55α co‐knockdown: (E) Cell viability (CCK‐8), (F) Mitotic catastrophe (α‐tubulin/DAPI), and (G) Paclitaxel response.

    Article Snippet: After blocking with 5% skimmed milk in TBST for 2 h at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies: anti‐MASTL (Cat# ab86387, Abcam), anti‐γ‐H2AX (Cat# 9718T, Cell Signaling Technology), anti‐Caspase‐2 (Cat# ET1602‐30, Huabio), anti‐Cleaved‐Caspase‐2 (Cat# R381055, Zenbio), anti‐ENSA (Cat# ab180513, Abcam), anti‐Phospho‐ENSA (Ser67) (Cat# 5240S, Cell Signaling Technology), anti‐PP2A‐B55α (Cat# A24261 , ABclone), and anti‐E2F1 (Cat# 66515‐1‐Ig, Proteintech).

    Techniques: Expressing, Activity Assay, Modification, Biomarker Discovery, Knockdown, CCK-8 Assay

    Mast2 (294–1197) robustly phosphorylates S67-ENSA. A, the site-specific information of selected significantly upregulated peptides from B ( green box ). B, nonradiolabeled substrate assay of ENSA (MCE) phosphorylated by Mast2 (294–1197). Total ENSA and FLAG immunoblot are shown with a representative pS67-ENSA blot ( right ), and phosphorylation was quantified by percent maximal AU with each aligned data point shown over a nonlinear curve determination ( left ). K m was calculated using GraphPad Prism. MAST, microtubule-associated serine/threonine; ENSA, endosulfine-α.

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of Mast2 kinase defines structural features, regulation, and substrates

    doi: 10.1016/j.jbc.2025.110922

    Figure Lengend Snippet: Mast2 (294–1197) robustly phosphorylates S67-ENSA. A, the site-specific information of selected significantly upregulated peptides from B ( green box ). B, nonradiolabeled substrate assay of ENSA (MCE) phosphorylated by Mast2 (294–1197). Total ENSA and FLAG immunoblot are shown with a representative pS67-ENSA blot ( right ), and phosphorylation was quantified by percent maximal AU with each aligned data point shown over a nonlinear curve determination ( left ). K m was calculated using GraphPad Prism. MAST, microtubule-associated serine/threonine; ENSA, endosulfine-α.

    Article Snippet: Primary antibodies for total AKT (2920S), phospho-specific AKT (pT308, 9275S and pS473, 4060L), phospho-specific S6K (pT389, 9205S), phospho-specific ENSA (pS67, 5240S), and total ENSA (8770S) were from Cell Signaling.

    Techniques: Western Blot, Phospho-proteomics